Su transmisión está mediada por semilla vegetativa, el vector Trialeurodes vaporariorum e injertos. Este virus también infecta tomate y puede permanecer en plantas asintomáticas. Key words: tripartite genome, Crinivirus, Solanum tuberosum, negative selection.Įl virus Potato yellow vein virus (PYVV), en español virus del amarillamiento de las venas de la papa, es el agente causal de la enfermedad conocida como amarillamiento de venas de la papa y puede reducir la producción hasta un 50%. The Tajima and Fu and Li tests revealed that these genes are subject to negative selection. In addition, the variability analysis based on maximum likelihood trees, haplotypes, and diversity indices showed that P26 is more variable than P10 and both are more variable in Andigena than in Phureja potatoes. The variability in these genes is influenced by the flow and uncontrolled use of vegetative seed between different provinces, that favor the dispersion of viral variants. We included sequences of each gene of the PYVV genome of potato and tomato isolates from GenBank. We analyzed the variability of P26 and P10 from 45 and 48 sequences, which were obtained by RT-PCR amplification of the total RNA of symptomatic potato leaves from the provinces of Nariño, Cundinamarca, and Boyaca (Colombia). Its genome has the P26 and P10 genes that are orthologues in the Crinivirus genus, which have been characterized as pathogenic factors and have not been studied in PYVV. PYVV transmission is mediated by vegetative seed, the vector Trialeurodes vaporariorum, and grafts. This virus also infects tomatoes and can remain asymptomatic in plants. Potato yellow vein virus (PYVV) is the causal agent of the potato yellow vein disease and can reduce potato production up to 50%. 108125 (More Nutritious Potatoes Project). Funding source: International Development Research Centre (IDRC)įunding source: Global Affairs Canada (GAC)įunding statement: The authors thank the financial support of the International Development Research Centre (IDRC) and Global Affairs Canada (GAC), through the Canadian International Food Security Research Fund (CIFSRF), which funded the Project Scaling up the Production of More Nutritious Yellow Potatoes in Colombia No.

Amplifx pcr